Electron Microscope Tomography and Single-particle Reconstruction | Cell Bio | Video - Preview

Watch the full video at https://www.jove.com/v/13405?utm_sour... Explore our full cell biology video textbook at https://www.jove.com/science-educatio... JoVE is the world-leading producer and provider of science videos with a mission to accelerate scientific research and education. Millions of scientists, educators and students across 1500+ High Schools, colleges and universities worldwide use JoVE’s library of 17,000+ videos for teaching, learning and research. JoVE High Schools offers expert-created video textbooks, lab videos, science experiment videos, interactive assessments, and seamless integration into school systems. These comprehensive learning resources help educators tackle teaching challenges, engage students, and meet curriculum requirements, enhancing the high school learning experience. Subscribe to our YouTube Channel -    / @jovehighschools   Follow us on - Facebook -   / jovehighschools   Instagram -   / jovehighschools   The 3D structure of biological samples can be determined using two cryo-electron microscopy techniques — electron tomography and single-particle reconstruction. In electron tomography, a sample is progressively tilted relative to the electron beam, capturing a series of 2D images from different angles. These 2D images are then stacked using computer software to generate the 3D image or tomogram of the sample.