Preparation of Samples for Electron Microscopy | Cell Bio | Video Textbooks - Preview

Watch the full video at https://www.jove.com/v/13402?utm_sour... Explore our full cell biology video textbook at https://www.jove.com/science-educatio... JoVE is the world-leading producer and provider of science videos with a mission to accelerate scientific research and education. Millions of scientists, educators and students across 1500+ High Schools, colleges and universities worldwide use JoVE’s library of 17,000+ videos for teaching, learning and research. JoVE High Schools offers expert-created video textbooks, lab videos, science experiment videos, interactive assessments, and seamless integration into school systems. These comprehensive learning resources help educators tackle teaching challenges, engage students, and meet curriculum requirements, enhancing the high school learning experience. Subscribe to our YouTube Channel -    / @jovehighschools   Follow us on - Facebook -   / jovehighschools   Instagram -   / jovehighschools   Biological samples must be prepared for electron microscopy to protect the sample from damage by the powerful electron beam and the low pressure created by the vacuum. Sample preparation starts with chemical fixation and complete dehydration. Chemical fixatives such as glutaraldehyde or formaldehyde cross-link the proteins, while osmium tetroxide is often used to fix the membrane lipids.