Single Particle Cryo-Electron Microscopy Application | Protocol Preview

Watch the Full Video at https://www.jove.com/v/62415/single-p.... Single Particle Cryo-Electron Microscopy: From Sample to Structure - a 2 minute Preview of the Experimental Protocol Joshua B.R. White, Daniel P. Maskell, Andrew Howe, Martin Harrow, Daniel K. Clare, C. Alistair Siebert, Emma L. Hesketh, Rebecca F. Thompson University of Leeds, Astbury Centre Structural Molecular Biology, School Molecular and Cellular Biology, Faulty Biological Sciences; Diamond Light Source, Harwell Science and Innovation Campus,; Structure determination of macromolecular complexes using cryoEM has become routine for certain classes of proteins and complexes. Here, this pipeline is summarized (sample preparation, screening, data acquisition and processing) and readers are directed towards further detailed resources and variables that may be altered in the case of more challenging specimens. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments