WB Flow Cytometry Analysis for Characterizing Monocyte Subset | Protocol Preview

Watch the Full Video at https://www.jove.com/v/57941/characte.... Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis - a 2 minute Preview of the Experimental Protocol Rekha Marimuthu, Habib Francis, Suat Dervish, Stephen C.H. Li, Heather Medbury, Helen Williams Westmead Hospital, Department of Surgery, Vascular Biology Research Centre; The University of Sydney, Westmead Clinical School, Department of Surgery; Westmead Institute for Medical Research, Westmead Research Hub; Westmead Hospital, Institute for Clinical Pathology and Medical Research; Here we present a protocol for characterizing monocyte subsets by whole blood flow cytometry. This includes outlining how to gate the subsets and assess their expression of surface markers and giving an example of the assessment of the expression of M1 (inflammatory) and M2 markers (anti-inflammatory). Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments