FC Analysis of Polarization of M1 and 2 Monocyte derived Cells | Protocol Preview

Watch the Full Video at https://www.jove.com/v/61807/polariza.... Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection - a 2 minute Preview of the Experimental Protocol Akhirunnesa Mily, Sadaf Kalsum, Marco Giulio Loreti, Rokeya Sultana Rekha, Jagadeeswara Rao Muvva, Magda Lourda, Susanna Brighenti Karolinska Institutet, Center for Infectious Medicine (CIM), Department of Medicine Huddinge, ANA Futura; International Centre for Diarrhoeal Disease Research, Bangladesh, Infectious Diseases Division; Karolinska Institutet, Clinical Microbiology, Department of Laboratory Medicine (Labmed), ANA Futura; Karolinska Institutet, Childhood Cancer Research Unit, Department of Women's and Children's Health; This protocol provides a method to study Mycobacterium tuberculosis infection in human M1- or M2-polarized macrophages based on differentiation of peripheral-blood-monocytes to macrophage-like cells that are infected with the GFP-labeled virulent strain H37Rv, and analyzed with flow cytometry using a 10-color panel including expression of selected M1/M2 markers. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments