Intravital Microscopy to visualize Macrophage Lytic Cell death | Protocol Preview

Watch the Full Video at https://www.jove.com/v/60698/visualiz.... Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy - a 2 minute Preview of the Experimental Protocol Liangfei Niu, Cong Wang, Kaile Zhang, Miaomiao Kang, Rui Liang, Xiaonan Zhang, Bo Yan Fudan University, Shanghai Public Health Clinical Center; Bengbu Medical College, School of Life Sciences; This protocol describes a technique for visualizing macrophage behavior and death in embryonic zebrafish during Mycobacterium marinum infection. Steps for the preparation of bacteria, infection of the embryos, and intravital microscopy are included. This technique may be applied to the observation of cellular behavior and death in similar scenarios involving infection or sterile inflammation. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments