Extracellular Trap Release in Macrophages | Protocol Preview

Watch the Full Video at https://www.jove.com/v/60541/in-vitro.... In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages - a 2 minute Preview of the Experimental Protocol Yunjia Zhang, Benjamin S. Rayner, Mathias Jensen, Clare L. Hawkins Heart Research Institute,; University of Sydney, Sydney Medical School; University of Copenhagen, Department of Biomedical Sciences; Presented here is a protocol to detect macrophage extracellular trap (MET) production in live cell culture using microscopy and fluorescence staining. This protocol can be further extended to examine specific MET protein markers by immunofluorescence staining. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments