Chemical Identification Test for Protein = GPAT Exclusive

Download "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Test and other GPAT Materials - https://play.google.com/store/apps/de... Test of Protein 1. Biuret test This test is used to detect the presence of peptide bond. When treated with copper sulphate solution in presence of alkali (NaOH or KOH), protein reacts with copper (II) ions to form a violet coloured complex called biuret. 2. Xanthoproteic test It is an identification test of protein and it gives a positive result with those proteins with amino acid carrying aromatic group. When protein is treated with hot concentrated nitric acid, a yellow coloured substance is formed. The yellow colour is due to xanthoproteic acid which is formed by the nitration of certain amino acids present in protein such as tyrosine and tryptophan. 3.Ninhydrin test This is a test for amino acids and proteins with free –NH2 group. When such an –NH2 group reacts with ninhydrin, an intense blue coloured complex is formed. 4. Millon’s test When egg albumin is treated with Millon’s reagent, it first gives a white coloured precipitate which then changes to brick red on boiling. Gelatin does not give this test. Get in touch with the solution by just clicking following links- Facebook Group-   / solutionpharamcy   Facebook Page-   / pharmavideo   New Channel (Pharmacy Dictionary)    / @pharmacydictionary   Instagram-   / solutionpharmacy   E-Mail for official and other work - [email protected] LinkedIn-   / pushpendrakpatel   #solutionpharmacy #Pharmacologyclass #Pharmacognosyvideos #GPATonlinetest #GPATclass #GPATvideos #Microbiologyclass#Microbiology#