OHISHI'S SOLUTION Reagent: Methylene blue .5% Acetone Sodium citrate Distilled water Ratio: 1cc of oxalated blood to 9cc of ohishi solution Procedure: 1. Mix oxalated blood and ohishi solution 2. Centrifuge for 10 minutes 3. Pour off the supernatant portion 4. Break the precipitate and place in the slide cover with cover slip Read under microscope using low power objective Look for microfilaria / makatidogandc http://www.makatidogandcathospital.com/ http://makatidogandcathosp.com/ http://makatidogandcathosp.com/