This talk covers the following topics in HIV Prevention from the CROI 2020 conference: Initiating PrEP during acute HIV infection Risk for ARV drug resistance Diagnostic and therapeutic challenges arise with early HIV infection on PrEP Near-perfect accuracy of a real-time urine tenofovir test compared to lab-based ELISA Nondaily use of HIV preexposure prophylaxis in a large online sample in the US Impact of PrEP and TasP on incidence of HIV diagnoses in 48 highest-burden US areas Development of a PrEP equity index to set local targets for PrEP coverage