Optogenetic Approach for Assessing Formation of Neuronal Connections | Protocol Preview

Watch the Full Video at https://www.jove.com/v/52408/an-optog.... An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System - a 2 minute Preview of the Experimental Protocol Colin T. E. Su, Su-In Yoon, Guillaume Marcy, Eunice W. M. Chin, George J. Augustine, Eyleen L. K. Goh Duke-NUS Graduate Medical School, Neuroscience & Behavioral Disorders; Nanyang Technological University, Lee Kong Chian School of Medicine; A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments