Reducing sugar test. Benedict's Reduction test by mbbs star#medical #neet2023 #mbbs #biochem lab

Reducing sugar test. Benedict's Reduction test by mbbs star#medical #neet2023 #mbbs #biochem lab What is reduced in Benedict's test? The copper (II) ions in the Benedict's solution are reduced to Copper (I) ions, which causes the color change. The red copper(I) oxide formed is insoluble in water and is precipitated out of solution. This accounts for the precipitate formed. What is the principle for Benedict's test? Benedict's test is based on the principle that under alkaline conditions reducing sugar forms enediols which are powerful reducing agents. The benedict solution contains milder alkali, Na2CO3 to maintain alkaline conditions. Reducing sugar which is changed into enediols reduces the cupric ions to cuprous ions. Why is it called Benedict's test? Benedict's reagent (also called Benedict's solution or Benedict's test) is a chemical reagent named after an American chemist, Stanley Rossiter Benedict. Benedict's reagent is used as a test for the presence of all monosaccharides, and generally also reducing sugars What is in Benedict's reagent? Principle of Benedict's Test The presence of the alkaline sodium carbonate converts the sugar into a strong reducing agent called enediols. During the reduction reaction, the mixture will change its color from blue to brick-red precipitate due to the formation of cuprous oxide (Cu2O).