Leukodepletion Filters Cells As Cell Source-Megakaryocyte Differentiation l Protocol Preview

Watch the Full Video at https://www.jove.com/v/62499/leukodep.... Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation - a 2 minute Preview of the Experimental Protocol Anaïs Pongerard, Lea Mallo, Christian Gachet, Henri de La Salle, François Lanza, Catherine Strassel Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS; This protocol describes in detail all the steps involved in obtaining leukofilter-derived CD34+ hematopoietic progenitors and their in vitro differentiation and maturation into proplatelet-bearing megakaryocytes that are able to release platelets in the culture medium. This procedure is useful for in-depth analysis of cellular and molecular mechanisms controlling megakaryopoiesis. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments