After viewing this video the audience should be able to describe the differences in measuring human chorionic gonadotropin (hCG) in serum versus urine. Attendees will be able to discuss the time line for positive results for different types of pregnancy tests. hepatocellular injury from biliary tract disease. The audience will be able to summarize the steps of a lateral flow assay commonly used in over-the-counter pregnancy tests. Viewers will be able to explain hemolytic diseases of the fetus and newborns and how passive immunity is used to prevent erythroblastosis fetalis. Key words: clinical chemistry, laboratory medicine, clinical pathology, pregnancy tests, trophoblastic disease, ectopic pregnancy, Robert, Fitzgerald