DNA Replication Process | All Enzymes & Steps Explained | Class 12 Biology | NEET | Easy explanation

In today's video, we decode the complete Process of DNA Replication. We have already proved that DNA replicates semi-conservatively. Now, it's time to understand the Machinery behind it. Who are the workers? How does the construction happen? I will explain the role of all 7 major enzymes (Helicase, Topoisomerase, Primase, Polymerase I & III, Ligase) and how they work together to copy DNA. We will also cover the concept of Leading vs. Lagging Strand and Okazaki Fragments using detailed diagrams. Topics Covered in This Video: The Machinery: Role of Helicase, Topoisomerase, SSB, Primase, Polymerase, and Ligase. Step-by-Step Process: Initiation at Origin, Unwinding, and Elongation. Leading vs. Lagging Strand: Why is one continuous and the other discontinuous? Okazaki Fragments: Formation and joining by Ligase. Primer Removal: The role of DNA Polymerase I. Why does DNA Polymerase only work in the 5' → 3' direction? Difference between DNA Polymerase I and III. Why are RNA primers needed for DNA replication? Function of Topoisomerase (DNA Gyrase). DNA replication process step by step Enzymes involved in DNA replication class 12 Leading and lagging strand difference What are Okazaki fragments? Function of DNA polymerase 1 and 3 Molecular basis of inheritance neet notes #NEET2026 #Class12Biology #DNAReplication #Enzymes #OkazakiFragments #BiologyNCERT #NEETPreparation #FutureDoctors #MolecularBasisOfInheritance